Raw potatoes and liver will act as the tissue samplescatalase activity will be estimated based on the amount of oxygen bubbles produced 1 package raw beef or chicken liver 2-3 large raw baking potatoes. Enzyme action: testing catalase activity (o 2) advanced biology with vernier 2 - 3 (o 2) figure 1 5 when data collection has finished, remove the o 2 gas sensor from the nalgene bottle. Vegetable & fruit sources of catalase by cathryn whitehead catalase is a critical protein found in the cells of nearly all organisms that are exposed to oxygen. Catalase is an enzyme, a biological (organic) catalyst hydrogen peroxide is the substrate for catalase the general procedure for the lab is outlined below, and specific details for each.
The materials needed for exercise 2a of the lab are: 30 ml of 15% (044 m) h2o2, a 50- ml glass beaker, 6 ml of freshly made catalase solution, a test tube, boiling water bath, 1 cm³ of liver, a knife for maceration, paper towels, safety goggles, lab apron, pencil, eraser, and paper to record results. The effect of substrate concentration on the activity of the enzyme catalase a level biology project aims this is an experiment to examine how the concentration of the substrate hydrogen peroxide affects the rate of reaction of the enzyme catalase. Page 1/11 teacher information sheet testing for catalase activity taken from ift experiments in food science series the chemical reactions that occur in living cells are complex and highly controlled. Potato enzyme lab essay sample an enzyme is a protein that speeds up or slows down a specific chemical reaction in an organism a good rule of thumb is to remember that enzyme names end in -ase.
Catalase high school molecular biology the structure and function of enzymes is a central theme in cellular and molecular biology in this laboratory exercise, a crude cell extract is prepared from potatoes. This lab allows us to observe the conversion of hydrogen peroxide (h2o2) into water and oxygen gas an enzyme known as catalase facilitates this decomposition reaction. Unformatted text preview: potato enzyme catalyst lab sophie sweeney ap biology 11/18/2015 question: what affects enzyme activityhypothesis: if the temperature of the catalase (potato) is raised, then the rate of reaction, (in floats per second), of the enzyme activity of hydrogen peroxide will increase because enzyme activity generally heightens as the temperature rises. Bio10 lab 3 enzymes and ph 31 6 mix the rennin and milk to start the enzyme reactions a with a clean squeeze bulb, put 3 drops of rennin solution from the cold tube of rennin into the cold milk. Potato enzyme lab analysis from looking at the graphs, it was obvious that since there was a larger surface area in condition one, that there were more bubbles produced presented and created by kevin greaves, makayla latham, aaron kuhn and alyssa rowse.
The enzyme catalase is found in many tissues including potato and liver it is important because it breaks down the hydrogen peroxide formed in metabolism hydrogen peroxide is toxic and if it were not broken down it would kill the cells. Catalase lab report investigating the effect of different environmental factors on the reaction of liver and hydrogen peroxide table of contents 1 design3 1 1 variables3 1 2 safety and environment3 2 data collection and analysis3 2. Ph procedure 1 use tape to label a test tube ap for acid & potato puree use a spatula to take a small amount (about 5 chunks) of your potato puree and transfer it into the ap test tube. Read this essay on how substrate concentration affects a catalase enzyme reaction come browse our large digital warehouse of free sample essays get the knowledge you need in order to pass your classes and more.
Catalase is an enzyme that is found in all cells this means that it is an intracellular enzyme and enzyme is a biological accelerator a accelerator is some thing that speeds up a reaction without being changed itself. Vi conclusion: this lab was conducted to find out the activity of enzyme catalase in different conditions: different ph, different temperature and different surface area in the experiment, control groups were set for comparing how changing environments could affect the activity of enzyme catalase. Catalase seems to work best in a basic environment, too much base (or acid) will denature the protein it is more important that students provide evidence from their experiment to support their claims.
Catalase lab report as the catalase and the substrate interact, the substrate is brought into the catalase and broken apart into water and oxygen this process may be able to happen naturally, but the enzyme, catalase, speeds up the process. The actual definition for hypotonic is: less concentrated hypertonic is the last out of the three words a cell in a hypertonic solution makes the water leave the cell to try to spread out the solutes outside to eventually reach an equillibrium. The decomposition of hydrogen peroxide by catalase is regarded as involving two reactions, namely, the catalytic decomposition of hydrogen peroxide, which is a maximum at the optimum ph 68 to 70, and the induced inactivation of catalase by the nascent oxygen produced by the hydrogen peroxide and still adhering to the catalase surface. Students can keep the size of the potato/liver the same and change the amount of liquid, and test to see how long it takes for the reaction to stop (when there are no more bubbles) 7) the potato because there are few enzymes and it continues until the break down of the h2o2 is complete.